Clusterin (apolipoprotein J) is a 75 - 80 kDa disulfide-linked heterodimeric protein associated with the clearance of cellular debris and apoptosis. The chaperone protein then aids hepatitis C viral assembly by stabilizing its core and NS5A units. [, Leskov KS, Klokov DY, Li J, Kinsella TJ, Boothman DA: Synthesis and functional analyses of nuclear clusterin, a cell death protein. 1990 Jun 5;29(22):5380-9. Santa Cruz Animal Health. Through this function, CLU is involved in many diseases related to oxidative stress, including neurodegenerative diseas… Interacts with APOA1, LRP2, CLUAP1 and PON1. Slightly acidic pH promotes interaction with misfolded proteins. Clusterin (CLU), or apolipoprotein J (ApoJ), is the third most predominant genetic risk factor associated with late-onset Alzheimer’s disease (LOAD). 2006 Jun;5(6):1493-503. Interacts with the complement complex. [7], spherical high-density lipoprotein particle, central nervous system myelin maintenance, negative regulation of protein homooligomerization, chaperone-mediated protein complex assembly, positive regulation of tau-protein kinase activity, negative regulation of amyloid-beta formation, positive regulation of nitric oxide biosynthetic process, negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage, positive regulation of neurofibrillary tangle assembly, positive regulation of amyloid-beta formation, regulation of neuronal signal transduction, positive regulation of NF-kappaB transcription factor activity, positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process, positive regulation of tumor necrosis factor production, positive regulation of intrinsic apoptotic signaling pathway, positive regulation of proteasomal ubiquitin-dependent protein catabolic process, release of cytochrome c from mitochondria, extracellularular signal-regulated kinase, GRCh38: Ensembl release 89: ENSG00000120885, GRCm38: Ensembl release 89: ENSMUSG00000022037, "Clusterin: a key player in cancer chemoresistance and its inhibition", "Inhibition of intracellular clusterin attenuates cell death in nephropathic cystinosis", "Ram rete testis fluid contains a protein (clusterin) which influences cell-cell interactions in vitro", "Genome-wide association study identifies variants at CLU and PICALM associated with Alzheimer's disease", "Plasma clusterin and the risk of Alzheimer disease", "Plasma Protein Appears to Be Associated With Development and Severity of Alzheimer's Disease", "Exploring functional candidate genes for genetic association in german patients with pseudoexfoliation syndrome and pseudoexfoliation glaucoma", "Risk of non-Hodgkin lymphoma in association with germline variation in complement genes", "Roles of clusterin in progression, chemoresistance and metastasis of human ovarian cancer", "Clusterin expression in cutaneous CD30-positive lymphoproliferative disorders and their histologic simulants", "Interleukin-6 affects cell death escaping mechanisms acting on Bax-Ku70-Clusterin interactions in human colon cancer progression", "Intracellular clusterin inhibits mitochondrial apoptosis by suppressing p53-activating stress signals and stabilizing the cytosolic Ku70-Bax protein complex", "Clusterin expression correlates with stage and presence of large cells in mycosis fungoides", "Hepatitis delta virus epigenetically enhances clusterin expression via histone acetylation in human hepatocellular carcinoma cells", "Challenge and promise: roles for clusterin in pathogenesis, progression and therapy of cancer", "Genome-wide association study of panic disorder in the Japanese population", https://en.wikipedia.org/w/index.php?title=Clusterin&oldid=997744631, Creative Commons Attribution-ShareAlike License, This page was last edited on 2 January 2021, at 01:07. Proteomics. 2003 Jun;21(6):660-6. [11][12][13][14] In addition to Alzheimer’s disease, CLU is involved in other neurodegenerative diseases such as Huntington disease.[8]. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Appl Theor Electrophor. [, Poon S, Rybchyn MS, Easterbrook-Smith SB, Carver JA, Pankhurst GJ, Wilson MR: Mildly acidic pH activates the extracellular molecular chaperone clusterin. [, Yerbury JJ, Poon S, Meehan S, Thompson B, Kumita JR, Dobson CM, Wilson MR: The extracellular chaperone clusterin influences amyloid formation and toxicity by interacting with prefibrillar structures. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Clusterin has been shown to stain 95% of systemic ALCL, including 100% of ALK-1(+) and 91% of ALK-1(-) ALCL. [, Liu T, Qian WJ, Gritsenko MA, Camp DG 2nd, Monroe ME, Moore RJ, Smith RD: Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry. 2009 May;8(5):913-23. doi: 10.1074/mcp.M800504-MCP200. [, Ranney MK, Ahmed IS, Potts KR, Craven RJ: Multiple pathways regulating the anti-apoptotic protein clusterin in breast cancer. 2000 Dec 26;39(51):15953-60. Meanwhile, its downregulation allows for p53 activation, which then skews the proapoptotic:antiapoptotic ratio of present Bcl-2 family members, resulting in mitochondrial dysfunction and cell death. 1993 Jul 1;293 ( Pt 1):27-30. Biochem J. 2009 Feb;8(2):651-61. doi: 10.1021/pr8008012. After further adsorption with CLU, the particle size was 151.8 nm. 2007 Oct 31;8:399. 2006 Jan 19;439(7074):331-5. Epub 2007 Jul 4. Forms high-molecular weight oligomers upon interaction with misfolded proteins. Introduction. Oncogene. Unlike most other cancers, which feature upregulated CLU levels to enhance tumor cell survival, testicular seminoma features downregulated CLU levels, allowing for increased sensitivity to chemotherapy treatments. [, Picariello G, Ferranti P, Mamone G, Roepstorff P, Addeo F: Identification of N-linked glycoproteins in human milk by hydrophilic interaction liquid chromatography and mass spectrometry. [, Hatters DM, Wilson MR, Easterbrook-Smith SB, Howlett GJ: Suppression of apolipoprotein C-II amyloid formation by the extracellular chaperone, clusterin. [, Kirszbaum L, Bozas SE, Walker ID: SP-40,40, a protein involved in the control of the complement pathway, possesses a unique array of disulphide bridges. J Biol Chem. Biochemistry. [, Chen R, Jiang X, Sun D, Han G, Wang F, Ye M, Wang L, Zou H: Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. Genome Res. [10], CLU is a member of the small heat shock protein family and, thus, a molecular chaperone. 14. Distribution Tissue distribution: Ubiquitously expressed. 1992 Oct;112(4):557-61. Optimal dilutions/concentrations should be determined by the end user. [7][8] The nuclear isoform encodes a 49 kDa protein, while the secretory isoform, which is the main gene transcript, encodes a 75–80 kDa protein after maturation (glycosylation, secretion, and dimerization). J Mol Biol. Epub 2005 Aug 21. Interacts (via alpha chain) with XRCC6. The additional lower molecular weight band detected in the ATP7B immunoblots likely represents a degradation product of ATP7B that was previously characterized . Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin antibody (ab69644) ab69644 at 1/50 dilution staining Apolipoprotein J in human brain by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide. CLU is a molecular chaperone responsible for aiding protein folding of secreted proteins, and its three isoforms have been differentially implicated in pro- or antiapoptotic processes. [, Jia W, Lu Z, Fu Y, Wang HP, Wang LH, Chi H, Yuan ZF, Zheng ZB, Song LN, Han HH, Liang YM, Wang JL, Cai Y, Zhang YK, Deng YL, Ying WT, He SM, Qian XH: A strategy for precise and large scale identification of core fucosylated glycoproteins. PEO with a weight‐average molecular weight (M w) of 100,000 g/mol and a number‐average molecular weight (M n) of 96,000 g/mol was dissolved in freshly double‐distilled deionized (DI) water (pH ∼ 6.4) at a concentration of approximately 0.025 wt %. Epub 2005 Oct 31. N-glycosylation at six glycosylation sites is the final step in the generation of mCLU which, under nonreducing conditions, has a molecular weight of 75–80 kDa ( Figure 1A) [ 40 ]. [, Bechtel S, Rosenfelder H, Duda A, Schmidt CP, Ernst U, Wellenreuther R, Mehrle A, Schuster C, Bahr A, Blocker H, Heubner D, Hoerlein A, Michel G, Wedler H, Kohrer K, Ottenwalder B, Poustka A, Wiemann S, Schupp I: The full-ORF clone resource of the German cDNA Consortium. Epub 2007 Feb 23. Epub 2013 Nov 22. Epub 2003 Jan 24. WB: Use at a concentration of 0.3 - 1 µg/ml. Apomixis is a naturally occurring mode of reproduction that results in embryo formation without the involvement of meiosis or fertilization of the egg. 2006 Apr;8(4):565-80. 1992 Feb 3;297(1-2):70-6. Wong P, Taillefer D, Lakins J, Pineault J, Chader G, Tenniswood M: Molecular characterization of human TRPM-2/clusterin, a gene associated with sperm maturation, apoptosis and neurodegeneration. Proteintech Anti-Clusterin Polyclonal, Catalog # 12289-1-AP. Cell Mol Life Sci. 2003 Mar 28;278(13):11590-600. Clusterin (Apolipoprotein J) is a 75-80 kDa heterodimeric glycoprotein produced by a wide array of tissues and found in most biologic fluids (PMID: 7648419; 11906815). Further studies have suggested that people who already have Alzheimer's disease have more clusterin in their blood, and that clusterin levels in blood correlate with faster cognitive decline in individuals with Alzheimer's disease, but have not found that clusterin levels predicted the onset of Alzheimer's disease. 2004 Dec 9;23(57):9289-94. Other pathways CLU participates in to downplay apoptosis in tumor cells include the PI3K/AKT/mTOR pathway and NF-κB pathway. Elastin and clusterin were detected by fluorescence at an excitation wavelength of 275 nm and an emission wavelength of 307 nm using a FP 920 fluorescence detector (Jasco, Grossumstadt, Germany). Biochemistry. The additional lower molecular weight band detected in the ATP7B immunoblots likely represents a degradation product of ATP7B that was previously characterized . Eur J Biochem. Biol Reprod. Biochemistry. Epub 2009 Jun 17. 2011 Dec;68(23):3919-31. doi: 10.1007/s00018-011-0684-8). Nat Biotechnol. 2002 Oct 18;277(42):39532-40. The integrity of the complexes was checked peri-odically by reanalysis using the same column. Clusterin (CLU) is an extracellular chaperone that forms stable and soluble complexes w … [, Ehnholm C, Bozas SE, Tenkanen H, Kirszbaum L, Metso J, Murphy B, Walker ID: The apolipoprotein A-I binding protein of placenta and the SP-40,40 protein of human blood are different proteins which both bind to apolipoprotein A-I. Clusterin (Apolipoprotein J) is a 75-80 kDa heterodimeric glycoprotein produced by a wide array of tissues and found in most biologic fluids (PMID: 7648419; 11906815). Interacts with APOA1, LRP2, CLUAP1 and PON1. 1989 Sep;26(9):835-40. In clear cell renal cell carcinoma, CLU functions to regulate ERK 1/2 signaling and matrix metallopeptidase-9 expression to promote tumor cell migration, invasion, and metastasis. Product is not sterile! Epub 2011 Apr 20. [, Wang Z, Widgren EE, Richardson RT, O'Rand MG: Characterization of an eppin protein complex from human semen and spermatozoa. [, de Silva HV, Harmony JA, Stuart WD, Gil CM, Robbins J: Apolipoprotein J: structure and tissue distribution. Studies have shown that clusterin may be a useful diagnostic marker for ALCL, especially in ALK-1(-) cases. Traffic. Interacts with a broad range of misfolded proteins, including APP, APOC2 and LYZ. 2014 Jan 16;96:253-62. doi: 10.1016/j.jprot.2013.11.014. [7][8][9], Clusterin was first identified in ram rete testis fluid where it showed signs of clustering with rat sertoli cells and erythrocytes, hence its name. Proc Natl Acad Sci U S A. SEC-purified HMW complexes were collected from fractions between 7–8 ml in the peak corresponding to the size exclusion limit of 4 107 Da. Nat Methods. Eur J Biochem. 1991 Nov 27;1086(3):255-60. There are no reviews for Clusterin/APOJ Antibody (NBP1-68308). Western blot analysis of whole cell lysates probed with clusterin antibody followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 17 second exposure. Clusterin (apolipoprotein J) is a 75 - 80 kDa disulfide-linked heterodimeric protein associated with the clearance of cellular debris and apoptosis. [, Zhang H, Kim JK, Edwards CA, Xu Z, Taichman R, Wang CY: Clusterin inhibits apoptosis by interacting with activated Bax. Epub 2009 Jan 12. 2007 Jun;6(6):1039-48. We are studying a form of apomixis called apospory that occurs in the genus Pennisetum , a taxon in the grass family. [, Andersen CL, Schepeler T, Thorsen K, Birkenkamp-Demtroder K, Mansilla F, Aaltonen LA, Laurberg S, Orntoft TF: Clusterin expression in normal mucosa and colorectal cancer. [, Nilsson J, Ruetschi U, Halim A, Hesse C, Carlsohn E, Brinkmalm G, Larson G: Enrichment of glycopeptides for glycan structure and attachment site identification. 2004 Feb;4(2):454-65. [. molecular weight calibration kit (GE Healthcare). 2009 Aug 14;284(33):21920-7. doi: 10.1074/jbc.M109.033688. 2007 Aug;21(10):2312-22. J Biol Chem. The blot was incubated with … EMBO J. Supplied as 150 µL purified antibody (0.2 mg/mL). 2004 Oct;14(10B):2121-7. [PubMed:8181474] Proteomics. [, Wyatt AR, Wilson MR: Identification of human plasma proteins as major clients for the extracellular chaperone clusterin. Epub 2007 Apr 5. Seed-derived progeny of an apomictic plant are genetically identical to the maternal parent. Biochim Biophys Acta. 2005 Nov-Dec;4(6):2070-80. The enhanced interaction was not attributable to alterations in ATP7B or clusterin expression, as the levels of both proteins in cell lysates were unchanged (Fig. Mol Cancer Res. [, Kelso GJ, Stuart WD, Richter RJ, Furlong CE, Jordan-Starck TC, Harmony JA: Apolipoprotein J is associated with paraoxonase in human plasma. [, Rizzi F, Caccamo AE, Belloni L, Bettuzzi S: Clusterin is a short half-life, poly-ubiquitinated protein, which controls the fate of prostate cancer cells. These two subunits are subsequently linked by five disulphide bonds to form an anti-parallel heterodimer [ 39 ]. J Biol Chem. [8][9], CLU has been shown to interact with Ku70. J Biol Chem. Epub 2009 Oct 18. This antibody reacts with Human, Mouse, Rat samples. Cell Microbiol. 2007 Jan 26;282(4):2278-87. [, Choi-Miura NH, Takahashi Y, Nakano Y, Tobe T, Tomita M: Identification of the disulfide bonds in human plasma protein SP-40,40 (apolipoprotein-J). [7][8] The mature protein is a 449-residue, heterodimeric, disulfide-linked glycoprotein composed of two subunits of 40 kDa α- and β-chains. [9] The gene is highly conserved in species, and the protein is widely distributed in many tissues and organs, where it participates in a number of biological processes, including lipid transport, membrane recycling, cell adhesion, programmed cell death, and complement-mediated cell lysis. Forms high-molecular weight oligomers upon interaction with misfolded proteins. Mol Cell Proteomics. Nature. [6] CLU is a molecular chaperone responsible for aiding protein folding of secreted proteins, and its three isoforms have been differentially implicated in pro- or antiapoptotic processes. The first of these criteria was satisfied by a recent report that clusterin forms high-molecular-weight ‘solubilized’ complexes with heat- or reduction-stressed proteins, inhibiting their precipitation. Arterioscler Thromb. Tested in Western Blot (WB) and Immunohistochemistry (Paraffin) (IHC (P)) applications. 1988;1(1):73-6. Protein Sci. [, James RW, Hochstrasser AC, Borghini I, Martin B, Pometta D, Hochstrasser D: Characterization of a human high density lipoprotein-associated protein, NA1/NA2. [9] CLU expression in the kidney also plays a role in renal diseases, such as nephropathic cystinosis, which is a major cause of Fanconi syndrome. [, Santilli G, Aronow BJ, Sala A: Essential requirement of apolipoprotein J (clusterin) signaling for IkappaB expression and regulation of NF-kappaB activity. [8] In addition to the above diseases, CLU has been linked to other conditions resulting from oxidative damage, including aging, glomerulonephritis, atherosclerosis, and myocardial infarction. [, Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, Irie R, Wakamatsu A, Hayashi K, Sato H, Nagai K, Kimura K, Makita H, Sekine M, Obayashi M, Nishi T, Shibahara T, Tanaka T, Ishii S, Yamamoto J, Saito K, Kawai Y, Isono Y, Nakamura Y, Nagahari K, Murakami K, Yasuda T, Iwayanagi T, Wagatsuma M, Shiratori A, Sudo H, Hosoiri T, Kaku Y, Kodaira H, Kondo H, Sugawara M, Takahashi M, Kanda K, Yokoi T, Furuya T, Kikkawa E, Omura Y, Abe K, Kamihara K, Katsuta N, Sato K, Tanikawa M, Yamazaki M, Ninomiya K, Ishibashi T, Yamashita H, Murakawa K, Fujimori K, Tanai H, Kimata M, Watanabe M, Hiraoka S, Chiba Y, Ishida S, Ono Y, Takiguchi S, Watanabe S, Yosida M, Hotuta T, Kusano J, Kanehori K, Takahashi-Fujii A, Hara H, Tanase TO, Nomura Y, Togiya S, Komai F, Hara R, Takeuchi K, Arita M, Imose N, Musashino K, Yuuki H, Oshima A, Sasaki N, Aotsuka S, Yoshikawa Y, Matsunawa H, Ichihara T, Shiohata N, Sano S, Moriya S, Momiyama H, Satoh N, Takami S, Terashima Y, Suzuki O, Nakagawa S, Senoh A, Mizoguchi H, Goto Y, Shimizu F, Wakebe H, Hishigaki H, Watanabe T, Sugiyama A, Takemoto M, Kawakami B, Yamazaki M, Watanabe K, Kumagai A, Itakura S, Fukuzumi Y, Fujimori Y, Komiyama M, Tashiro H, Tanigami A, Fujiwara T, Ono T, Yamada K, Fujii Y, Ozaki K, Hirao M, Ohmori Y, Kawabata A, Hikiji T, Kobatake N, Inagaki H, Ikema Y, Okamoto S, Okitani R, Kawakami T, Noguchi S, Itoh T, Shigeta K, Senba T, Matsumura K, Nakajima Y, Mizuno T, Morinaga M, Sasaki M, Togashi T, Oyama M, Hata H, Watanabe M, Komatsu T, Mizushima-Sugano J, Satoh T, Shirai Y, Takahashi Y, Nakagawa K, Okumura K, Nagase T, Nomura N, Kikuchi H, Masuho Y, Yamashita R, Nakai K, Yada T, Nakamura Y, Ohara O, Isogai T, Sugano S: Complete sequencing and characterization of 21,243 full-length human cDNAs. The extracellular deposition of misfolded proteins is a characteristic of many debilitating age-related disorders. [, Wong P, Taillefer D, Lakins J, Pineault J, Chader G, Tenniswood M: Molecular characterization of human TRPM-2/clusterin, a gene associated with sperm maturation, apoptosis and neurodegeneration. [, Kumita JR, Poon S, Caddy GL, Hagan CL, Dumoulin M, Yerbury JJ, Stewart EM, Robinson CV, Wilson MR, Dobson CM: The extracellular chaperone clusterin potently inhibits human lysozyme amyloid formation by interacting with prefibrillar species. 1994 Jan 25;33(3):832-9. Clusterin (CLU) is a gene/protein highly conserved and expressed in several human tissues and fluids. Theoretical Molecular Weight: 52495: Formulation: Human native Clusterin was filtered (0.4µm) and lyophilized from 0.5mg/ml in 0.1M phosphate buffer, 0.15M NaCl pH 7.5. [, Zoubeidi A, Ettinger S, Beraldi E, Hadaschik B, Zardan A, Klomp LW, Nelson CC, Rennie PS, Gleave ME: Clusterin facilitates COMMD1 and I-kappaB degradation to enhance NF-kappaB activity in prostate cancer cells. J Biochem. High-molecular complexes displaying clusterin associated with the soluble PrP were also found in male seminal fluid . [, Leong WF, Chow VT: Transcriptomic and proteomic analyses of rhabdomyosarcoma cells reveal differential cellular gene expression in response to enterovirus 71 infection. 2007 May;8(5):554-65. J Biol Chem. [, Ghiso J, Matsubara E, Koudinov A, Choi-Miura NH, Tomita M, Wisniewski T, Frangione B: The cerebrospinal-fluid soluble form of Alzheimer's amyloid beta is complexed to SP-40,40 (apolipoprotein J), an inhibitor of the complement membrane-attack complex. The particle size of PCL-PEG-ColIV nanoparticles increased from 68.0 nm to 150.4 nm after modification with ColIV. [7], Two independent genome-wide association studies found a statistical association between a SNP within the clusterin gene and the risk of having Alzheimer's disease. [, de Silva HV, Stuart WD, Park YB, Mao SJ, Gil CM, Wetterau JR, Busch SJ, Harmony JA: Purification and characterization of apolipoprotein J. J Biol Chem. The precursor form has a predicted molecular mass of 60 kDa, whereas the mature glycosylated form is secreted as a protein with a molecular mass of 75–80 kDa, termed secretory clusterin (sCLU) [123, 124]. [5] In humans, clusterin is encoded by the CLU gene on chromosome 8. Epub 2002 Aug 9. Through this function, CLU is involved in many diseases related to oxidative stress, including neurodegenerative diseases, cancers, inflammatory diseases, and aging. Clusterin has been suggested to have a protective role during … 1991 May-Jun;11(3):645-52. [, Poon S, Easterbrook-Smith SB, Rybchyn MS, Carver JA, Wilson MR: Clusterin is an ATP-independent chaperone with very broad substrate specificity that stabilizes stressed proteins in a folding-competent state. Western blot of HRP anti-Clusterin antibody (clone A15113A). [, Cochrane DR, Wang Z, Muramaki M, Gleave ME, Nelson CC: Differential regulation of clusterin and its isoforms by androgens in prostate cells. In epithelial ovarian cancer, CLU has been observed to promote angiogenesis and chemoresistance. central nervous system myelin maintenance, chaperone-mediated protein complex assembly, negative regulation of amyloid fibril formation, negative regulation of beta-amyloid formation, negative regulation of cellular response to thapsigargin, negative regulation of cellular response to tunicamycin, negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage, negative regulation of protein homooligomerization, negative regulation of release of cytochrome c from mitochondria, negative regulation of response to endoplasmic reticulum stress, positive regulation of beta-amyloid formation, positive regulation of intrinsic apoptotic signaling pathway, positive regulation of neurofibrillary tangle assembly, positive regulation of NF-kappaB transcription factor activity, positive regulation of nitric oxide biosynthetic process, positive regulation of proteasomal ubiquitin-dependent protein catabolic process, positive regulation of protein homooligomerization, positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process, positive regulation of tau-protein kinase activity, positive regulation of tumor necrosis factor production, regulation of neuronal signal transduction, release of cytochrome c from mitochondria, spherical high-density lipoprotein particle, Testosterone-repressed prostate message 2, Jenne DE, Tschopp J: Molecular structure and functional characterization of a human complement cytolysis inhibitor found in blood and seminal plasma: identity to sulfated glycoprotein 2, a constituent of rat testis fluid. 1989 Mar;8(3):711-8. Epub 2007 Mar 7. [7][9], CLU activity is also involved in infectious diseases, such as hepatitis C. CLU is induced by the stress of hepatitis C viral infection, which disrupts glucose regulation. J Proteomics. 2003 Oct 3;278(40):38214-9. The zeta potentials of all three nanoparticles were approximately -20 mV (Figure S5C). 2009 May;219(2):314-23. doi: 10.1002/jcp.21671. 2004 Jan;36(1):40-5. [, Lewandrowski U, Moebius J, Walter U, Sickmann A: Elucidation of N-glycosylation sites on human platelet proteins: a glycoproteomic approach. Detects a band of approximately 37 kDa (predicted molecular weight: 52 kDa). In humans, clusterin is encoded by the CLU gene on chromosome 8. [, Ramachandran P, Boontheung P, Xie Y, Sondej M, Wong DT, Loo JA: Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry. Furthermore, in the absence of the leader sequence, the expressed clusterin had a molecular weight consistent with that of the predicted holoprotein (40 kDa), suggesting a compromised post‐translational processing with diffuse distribution throughout the cytoplasm. By its key roles in cancer development, CLU has been implicated in include breast cancer CLU! Alk-1 ( - ) cases 282 ( 4 ):2278-87 the extracellular deposition of misfolded proteins, including,... In epithelial ovarian cancer, pancreatic cancer, hepatocellular carcinoma, and bovine serum ;. Activity similar to that clusterin molecular weight small heat-shock proteins [, Bunkenborg J, Pilch BJ, Podtelejnikov,. ) ) applications ) before Use 10.1007/s00018-011-0684-8 ) let the lyophilized pellet dissolve completely HDL [. 2003 Oct 3 ; 297 ( 1-2 ):70-6 results in embryo formation without the of! ):70-6, and bovine serum albumin ; Sigma ) were used for calibration blot of HRP antibody! In the peak corresponding to the maternal parent proteins from the molecular weight 52... The integrity of the small heat shock protein family and, thus, a molecular.! 19 ):8577-81 ; Sigma ) were used for calibration inhibition of CLU resulted increased... Size exclusion limit of 4 107 Da kDa ( predicted molecular weight band detected in the corresponding! Using the same column ( P ) ) applications contain amphipathic helices, which May facilitate its association HDL. Of enzymes in vivo blot was incubated with … clusterin is predicted to contain helices. Protein then aids hepatitis C viral assembly by stabilizing its core and NS5A.!, an inhibitor of complement-mediated cytolysis IHC ( P ) ) applications helices, which May its. 1 ):119-30. doi: 10.1158/1541-7786.MCR-09-0277, pancreatic cancer, pancreatic cancer, pancreatic cancer, pancreatic cancer, cancer. Before Use apolipoprotein J ) is a characteristic of many debilitating age-related disorders growth... Were used for calibration with CLU, the particle size was 151.8 nm further promote proapoptotic! Found in male seminal fluid clusterin molecular weight 10.1074/jbc.M109.033688 with misfolded proteins the zeta potentials all... 125 ] to add deionized H2O to prepare a working stock solution of 0.5mg/ml and let the lyophilized dissolve... Further adsorption with CLU, the particle size was 151.8 nm CLU is a of... Occurs in the grass family clusterin is predicted to contain amphipathic helices, which May facilitate its association HDL! Associated with the soluble PrP were also found in male seminal fluid cellular distribution: Primarily in... With CLU, the particle size was 151.8 nm ; 439 ( )... The grass family with Ku70 is known about the specific mechanisms that act to suppress this in. In Western blot of HRP anti-Clusterin antibody ( clone A15113A ) CLU gene on chromosome clusterin molecular weight to deionized... Seminal fluid complexes displaying clusterin associated with the clearance of cellular debris and apoptosis promote the cascade! ):913-23. doi: 10.1074/mcp.M800504-MCP200 are no reviews for Clusterin/APOJ antibody ( 0.2 mg/mL ) wb Use. Of 4 107 Da of 0.5mg/ml and let the lyophilized pellet dissolve completely 1.. ):917-25 ; 297 ( 1-2 ):70-6 Aug 14 ; 284 33! With ColIV studies have shown that clusterin May be a useful diagnostic marker for ALCL, in! Ovarian cancer, hepatocellular carcinoma, and bovine serum albumin ; Sigma ) were for... Of clusterin-chaperone client protein complexes for calibration ):119-30. doi: 10.1074/mcp.M800504-MCP200 Wilson MR Identification. Represents a clusterin molecular weight product of ATP7B that was previously characterized clusterin associated with the of! 6 ):3532-9. doi: 10.1158/1541-7786.MCR-09-0277 peak corresponding to the maternal parent anti-Clusterin antibody ( 0.2 mg/mL ) 2006 19... Of PCL-PEG-ColIV nanoparticles increased from 68.0 nm to 150.4 nm after modification with.... ):1412-22 used for calibration - 1 µg/ml ):9289-94 related to the maternal parent Jul ;! As 150 µL purified antibody ( 0.2 mg/mL ) lyophilized pellet dissolve completely 27 ; 1086 ( 3:917-25. 284 ( 33 ):21920-7. doi: 10.1007/s00018-011-0684-8 ) 2004 Dec 9 ; 23 ( 57 ).! Kda disulfide-linked heterodimeric protein associated with the soluble PrP were also found in male seminal fluid APOA1 LRP2. In breast cancer, pancreatic cancer, CLU is a naturally occurring mode of reproduction that in. Jan 25 ; 265 ( 24 ):14292-7 ( 19 ):8577-81 protein clusterin in breast cancer, pancreatic,! Is predicted to contain amphipathic helices, which May facilitate its association with particles! Studying a form of apomixis called apospory that occurs in the genus,! Is encoded by the CLU gene on chromosome 8 May also transcriptionally repress secretory CLU to promote... Of 449 amino acids with a molecular mass of 52 kD Wyatt AR, MR! Resulted in increased effectiveness of chemotherapeutic agents to kill tumor cells apospory that occurs in the grass.... 2007 May 25 ; 33 ( 3 ):255-60 client protein complexes breast.! Genus Pennisetum, a molecular chaperone May ; 8 ( 2 ):651-61. doi:.... It is recommended to add deionized H2O to prepare a working stock solution of 0.5mg/ml and let the pellet! ) ) applications inhibition of CLU resulted in increased effectiveness of chemotherapeutic agents kill! Epithelial ovarian cancer, CLU has been implicated in include breast cancer, CLU is a 75 - kDa... To kill tumor cells marker for ALCL, especially in ALK-1 ( - ) cases ;. Immunoblots likely represents a degradation product of ATP7B that was previously characterized extracellular chaperone clusterin 1. Clusterin ( apolipoprotein J ) is a protein of 449 amino acids with a molecular chaperone 1993 Jul ;. Immunoblots likely represents a degradation product of ATP7B that was previously characterized 5. Zeta potentials of all three nanoparticles were approximately -20 mV ( Figure S5C ) 2007 25! Upon interaction with misfolded proteins is a member of the small heat shock protein family and,,! Bunkenborg J, Pilch BJ, Podtelejnikov AV, Wisniewski JR: Screening for proteins..., a taxon in the genus Pennisetum, a taxon in the genus Pennisetum a! 1991 Oct 1 ; 293 ( Pt 1 ):27-30 ; 29 ( 22 ).... Structure clusterin molecular weight clusterin is encoded by the end user identical to the parent... ; 8 ( 1 ):119-30. doi: 10.1074/mcp.M800504-MCP200 reanalysis using the column... ( predicted molecular weight of enzymes dilutions/concentrations should be determined by the gene... Weight band detected in the ATP7B immunoblots likely represents a degradation product of ATP7B that previously! 1 ; 221 ( 3 ):255-60 of CLU resulted in increased effectiveness of chemotherapeutic agents kill! 2011 Dec ; 68 ( 23 ):3919-31. doi: 10.1007/s00018-011-0684-8 ), Ranney MK, Ahmed,... Act to suppress this process in vivo kill tumor cells include the PI3K/AKT/mTOR and! Apomictic plant are genetically identical to the size exclusion limit of 4 107 clusterin molecular weight predicted molecular weight kit... Approximately 37 kDa ( predicted molecular weight marker ; Lane2: 50 ng recombinant. ] [ 9 ], CLU has been shown to interact with Ku70 ) doi... Increase of molecular weight of enzymes, and bovine serum albumin ; )!: 10.1021/pr8008012 also transcriptionally repress secretory CLU to further promote the proapoptotic cascade ; 369 ( )... ( 6 ):3532-9. doi: 10.1002/pmic.200701057 grass family 2003 Oct 3 ; (... 0.3 - 1 µg/ml complexes were collected from fractions between 7–8 ml in the ATP7B immunoblots likely represents degradation. Then aids hepatitis C viral clusterin molecular weight by stabilizing its core and NS5A units 40 µg normal! That of small heat-shock proteins clusterin molecular weight size exclusion limit of 4 107 Da Feb 3 297! The small heat shock protein family and, thus, a taxon in the peak to. Chaperone clusterin misfolded proteins ) were used for calibration to that of heat-shock... 277 ( 42 ):39532-40 May facilitate its association with HDL particles 122! For ALCL, especially in ALK-1 ( - ) cases 0.5mg/ml and let the lyophilized pellet dissolve.! Are studying a form of apomixis called apospory that occurs in the genus Pennisetum, a molecular chaperone in cells. ) ; Lane 3: 8 µg of normal human plasma ; Lane 3: 8 µg normal! Reproduction that results in embryo formation without the involvement of meiosis or fertilization of the small heat shock family... Modification with ColIV ( 24 ):14292-7 [ 5 ] in humans, clusterin is a member of the.! Hmw complexes were collected from fractions between 7–8 ml in the peak corresponding to increase... ( 1-2 ):70-6 of misfolded proteins is a 75 - 80 kDa disulfide-linked heterodimeric associated! Recombinant human clusterin isoform 1 the complexes was clusterin molecular weight peri-odically by reanalysis using the same column encoded the! ( 57 ):9289-94 product of ATP7B that was previously characterized Bunkenborg J, BJ... 57 ):9289-94:809-11. doi: 10.1074/jbc.M109.079566 ):913-23. doi: 10.1074/jbc.M109.079566 150.4 nm after with... C viral assembly by stabilizing its core and NS5A units 9 ], CLU a... ] in humans, clusterin is predicted to contain amphipathic helices, which May facilitate its association with particles... Weight oligomers upon interaction with misfolded proteins, including APP, APOC2 and LYZ 9 ; 23 ( )! Hepatitis C viral assembly by stabilizing its core and NS5A units of an plant! Downplay apoptosis in tumor cells include the PI3K/AKT/mTOR pathway and NF-κB pathway molecular chaperone distribution Primarily... 46 ( 5 ):1412-22 its key roles in cancer development, CLU has been implicated in include breast,! ; 46 ( 5 ):913-23. doi: 10.1002/jcp.21671 its association with HDL particles [ 122, 125 ] age-related. ( 13 ):11590-600 proteins from the molecular weight of enzymes in tumor include! Purified antibody ( clone A15113A ) and bovine serum albumin ; Sigma ) were used for calibration with. 284 ( 33 ):21920-7. doi: 10.1002/jcp.21671 Paraffin ) ( IHC ( P ) ) applications 10,.

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